Journal: bioRxiv
Article Title: Minimal Amino Acid Alphabet for Protein Design
doi: 10.64898/2026.03.06.710107
Figure Lengend Snippet: (A) Predicted 3D structure colored by pLDDT (top) or secondary structure/atom type (bottom). (B) Gel permeation chromatography elution profile (* indicates LAGVSTDP). (C) Electronic circular dichroism spectrum of LAGVSTDP. (D) Temperature denaturation profile of LAGVSTDP. R refers to a refolding attempt (cooling back to 20 °C after 90 °C).
Article Snippet: The pooled fractions containing the expressed protein were desalted by gel filtration (PD-10 columns packed with SephadexTM G-25 resin, Cytiva, in PBS) and loaded onto a liquid chromatography system (NGC Discover 10 Chromatography System, BioRad, USA) equipped with the gel permeation chromatography column (HiPrep 16/60 SephacrylTM S-100 HR, Cytiva).
Techniques: GPC Assay, Circular Dichroism